Yuma Shisaka

Personal History

• 03.2017 MSc in Chemistry | Department of Chemistry, Graduate School of Science, Nagoya University (Prof. Yoshihito Watanabe)
• 03.2020 PhD in Chemistry | Department of Chemistry, Graduate School of Science, Nagoya University (Prof. Osami Shoji)
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• 04.2018 JSPS Research Fellow (DC2)
• 04.2020 Postdoctoral Researcher (CREST Researcher) | Graduate School of Science, Nagoya University (Prof. Osami Shoji)
• 04.2021 JSPS Research Fellow (PD) | Centre for Sustainable Resource Science, RIKEN (Prof. Shinya Hagihara)
• 04.2024 Special Postdoctral Researcher | Centre for Sustainable Resource Science, RIKEN (Prof. Shinya Hagihara)

Keywords

• Protein engineering, Chemical biology, Protein structure,  Bioinorganic chemistry

Awards

• 07.2019 “Poster Award” NU-UoE JD and JSPS Core-to-Core Joint-Workshop
• 09.2018 “Poster Award” The 31st Summer Seminar of Bioinorganic Chemistry
• 04.2018 “CSJ Student Presentation Award 2018” The 98th CSJ Annual Meeting
• 06.2017 “Poster Award” The 17th Annual Meeting of the Protein Science Society of Japan
• 03.2017 “Graduate School of Science Award” Graduate School of Science, Nagoya University
• 03.2017 “Best Master Thesis Award” Graduate School of Science, Nagoya University
• 09.2016 “Poster Award” The 66th Japan Society of Coordination Chemistry
• 09.2016 “RSC Best Poster Presentation Award” The 10th Symposium on Biorelevant Chemistry
• 07.2016 “Student Best Poster Award” The 26th Symposium on Biopolymers Science
• 11.2015 “CSJ Poster Presentation Award 2015 for Excellent Research” The 5th CSJ Chemistry Festa

Publications

Original papers

8. Hiroaki Inaba, Yuma Shisaka, Shinya Ariyasu*, Erika Sakakibara, Garyo Ueda, Yuichiro Aiba, Nobutaka Shimizu, Hiroshi Sugimoto, Osami Shoji*, “Heme-Substituted Protein Assembly Bridged by Synthetic Porphyrin: Achieving Controlled Configuration while Maintaining Rotational Freedom” RSC Adv. 2024, 14, 8829–8836.
9. Shinya Ariyasu, Kai Yonemura, Chie Kasai, Yuichiro Aiba, Hiroki Onoda, Yuma Shisaka, Hiroshi Sugimoto, Takehiko Tosha, Minoru Kubo, Takashi Kamachi, Kazunari Yoshizawa, Osami Shoji* “Catalytic Oxidation of Methane by Wild-Type Cytochrome P450BM3 with Chemically Evolved Decoy Molecules” ACS Catal. 2023, 13, 8613–8623.
10. Kazuto Suzuki, Josha Kyle Stanfield, Keita Omura, Yuma Shisaka, Shinya Ariyasu, Chie Kasai, Yuichiro Aiba, Hiroshi Sugimoto* Osami Shoji* “A Compound I Mimic Reveals the Transient Active Species of a Cytochrome P450 Enzyme: Insight into the Stereoselectivity of P450-Catalysed Oxidations” Angew. Chem. Int. Ed. 2023, 62, e202215706.
11. Yuma Shisaka, Erika Sakakibara, Kazuto Suzuki, Joshua Kyle Stanfield, Hiroki Onoda, Garyo Ueda, Miu Hatano, Hiroshi Sugimoto, Osami Shoji* “Tetraphenylporphyrin Enters the Ring: First Example of a Complex Between Highly Bulky Porphyrins and a Protein” ChemBioChem 2022, 23, e202200095.
12. Kazuto Suzuki, Yuma Shisaka, Joshua Kyle Stanfield, Yoshihito Watanabe, Osami Shoji* “Enhanced cis- and enantioselective cyclopropanation of styrene catalysed by cytochrome P450BM3 using decoy molecules” Chem. Commun. 2020, 56, 11026–11029.
13. Yuma Shisaka, Yusuke Iwai, Shiho Yamada, Hiromu Uehara, Takehiko Tosha, Hiroshi Sugimoto, Yoshitsugu Shiro, Joshua Kyle Stanfield, Kazuya Ogawa, Yoshihito Watanabe, Osami Shoji* “Hijacking the Heme Acquisition System of Pseudomonas aeruginosa for the Delivery of Phthalocyanine as an Antimicrobial” ACS Chem. Biol. 2019, 14, 1637−1642.
14. Erika Sakakibara, Yuma Shisaka, Hiroki Onoda, Daiki Koga, Ning Xu, Toshikazu Ono, Yoshio Hisaeda, Hiroshi Sugimoto, Yoshitsugu Shiro, Yoshihito Watanabe, Osami Shoji* “Highly malleable haem-binding site of the haemoprotein HasA permits stable accommodation of bulky tetraphenylporphycenes” RSC Adv. 2019, 9, 18697−18702.
15. Hiromu Uehara, Yuma Shisaka, Tsubasa Nishimura, Hiroshi Sugimoto, Yoshitsugu Shiro, Yoshihiro Miyake, Hiroshi Shinokubo, Yoshihito Watanabe, Osami Shoji* “Structures of the Heme Acquisition Protein HasA with Iron(III)-5,15-Diphenylporphyrin and Derivatives Thereof as an Artificial Prosthetic Group” Angew. Chem. Int. Ed. 2017, 56, 15279−15283.

Review papers

6. Yuma Shisaka, Osami Shoji* “Bridging the gap: Unveiling novel functions of a bacterial haem-acquisition protein capturing diverse synthetic porphyrinoids” Coord. Chem. Rev. 2022, 472, 214793.
7. 四坂勇磨, 荘司長三 “「トロイの木馬」的戦略による緑膿菌の高選択的緑膿菌” 第54回緑膿菌感染症研究会講演記録 1–5, 2020.
8. 荘司長三, 四坂勇磨 “人工金属タンパク質で緑膿菌を狙い撃ち ~緑膿菌が分泌するヘムタンパク質で金属フタロシアニンを送り込む~” ファルマシア 2020, 56(8), 749−753.
9. 荘司長三, 四坂勇磨 “鉄獲得系を乗っ取る緑膿菌の光殺菌 ~緑膿菌を騙して抗菌薬を取り込ませる新規手法~” 『化学』3月号, 2020, 75, 23−28.
10. 四坂勇磨, 荘司長三 “鉄の取り込みを阻害する新しい抗菌薬の開発” 感染症48, 12−13 & 18−21, 2018.
11. 四坂勇磨 “緑膿菌の細胞外ヘム獲得蛋白質 HasA を利用した外膜ヘム輸送体 HasR と不溶性金属錯体の複合体形成” Bull. Jpn. Soc. Coord. Chem. 2017, 69, 103−105.